PRODH antibody (AA 116-433)

Catalog No. ABIN7599773

This Rabbit Polyclonal antibody specifically detects PRODH in WB, ELISA, IHC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PRODH antibody (AA 116-433) (ABIN7599773)

Target: PRODH (Proline Dehydrogenase (Oxidase) 1 (PRODH))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRODH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-PRODH Antibody

Binding Specificity: AA 116-433

Purpose: Anti-PRODH Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PRODH Antibody Picoband® (ABIN7599773). Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PRODH recombinant protein (Position: H116-E433).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Afenjar, A., Moutard, M.-L., Doummar, D., Guet, A., Rabier, D., Vermersch, A.-I., Mignot, C., Burglen, L., Heron, D., Thioulouse, E., de Villemeur, T. B., Campion, D., Rodriguez, D. Early neurological phenotype in 4 children with biallelic PRODH mutations. Brain Dev. 29: 547-552, 2007. 2. Baron, M. Genetics of schizophrenia and the new millennium: progress and pitfalls. Am. J. Hum. Genet. 68: 299-312, 2001. 3. Bender, H.-U., Almashanu, S., Steel, G., Hu, C.-A., Lin, W.-W., Willis, A., Pulver, A., Valle, D. Functional consequences of PRODH missense mutations. Am. J. Hum. Genet. 76: 409-420, 2005.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PRODH

Target: PRODH (Proline Dehydrogenase (Oxidase) 1 (PRODH))

Alternative Name: PRODH

Background:

Synonyms: Peptidyl-prolyl cis-trans isomerase B, PPIase B, 5.2.1.8, CYP-S1, Cyclophilin B, Rotamase B, S-cyclophilin, SCYLP, PPIB, CYPB,

Tissue Specificity: Found mainly in the liver, kidney and skeletal muscle.

Background: Proline dehydrogenase, mitochondrial is an enzyme that in humans is encoded by the PRODH gene. This gene encodes a mitochondrial protein that catalyzes the first step in proline degradation. Mutations in this gene are associated with hyperprolinemia type 1 and susceptibility to schizophrenia 4 (SCZD4). This gene is located on chromosome 22q11.21, a region which has also been associated with the contiguous gene deletion syndromes, DiGeorge and CATCH22. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 69 kDa

Gene ID: 5625

UniProt: O43272

Pathways: Response to Water Deprivation, Monocarboxylic Acid Catabolic Process